First, the raw material handling: Fresh pig lung (or frozen pig lung natural thawing) carefully washed with water and wash dirt and external dirt and external skin fat, minced into minced, and with full mixing, adding an equal amount of After mixing with water, add a small amount of preservative to dissolve them evenly.

  Second, enzymatic extraction: First, the above raw materials under full mixing, with a small amount of dilute lye finely adjusted to a PH value of 8-9 (available with the corresponding precision PH test strips, the same below) before adding minced Fresh pancreatic juice as digestible (added pig pancreas according to the actual weight of the raw material solution of 1% -1.5% is appropriate), stir, slowly warming to about 40 degrees, continue stirring and keep the feed solution PH = 7.5 -8, to maintain the liquid temperature at 37-40 degrees, enzymatic hydrolysis 3-4 hours, then warmed to 47-50 degrees, to maintain the PH value = 8.0-8.5, then add a little pork pancreas as appropriate, continue to digest 4 -5 hours, during the enzymolysis process, if the enzymatic hydrolysis of the PH paper review of the occasion of the occasion, the sentence should be promptly with a little dilute lye (5% -8% NaOH solution) carefully adjusted. Hydrochloric acid adjusted its pH = 5.5-6, and then heated to 80 degrees. Under full stirring, add about 5% of the total weight of the feed solution of refined salt (containing NaCL ≥ 95%, Calcium Magnesium Calcium Salt <0.5%), so that after mixing evenly, then heated 90 degrees, incubated for 30 minutes, Filtered while hot to remove impurities until the filtrate was cooled to 37 degrees, diluted with dilute alkaline solution to adjust its PH = 10.5, fine filtration, the filtrate was adjusted to pH = 9.0-9.5 range of ion exchange adsorption treatment.

  Third, the ion exchange adsorption; first the above enzyme solution was cooled to room temperature, carefully remove the floating in the liquid layer of fat sheet, control the temperature was raised to 45 degrees, stop heating, adding pre-mixing under good D-254 Type resin has been effective in the absorption of heparin sodium feedstock composition, the amount of new resin is generally 2.55-3% of the feed liquid, after the regeneration is known to be appropriate to increase its dosage, stirring, adsorption treatment 3 hours after standing Filtration, the filtrate can be used as the production of coronary heart disease new crown Corian Shu raw materials.

  The above-mentioned heparin component has been adsorbed D-254 resin first rinse thoroughly with water, clean, and then about double the 1.2mol / L sodium chloride solution washed D-254 resin heparin sodium operation: the first The secondary eluent is about 1.5 times the volume of the resin, about 4 hours for elution, about 0.5 times the volume of the second elution, and the elution time is 1 hour. The resin is filtered off and the eluate is combined. The eluate obtained above was adjusted to PH = 10-11, stirred for 30 minutes, allowed to stand for 6 hours, and then the supernatant was carefully precipitated. The lower precipitate was suction filtered to dryness as much as possible. Combine overnight and the filtrate, finely adjust PH = 6.0-6.5, add 1.5 times the amount of 95% ethanol precipitation overnight, the next day, carefully bake the supernatant, the lower part of the precipitate was collected and suction filtered dry (mother liquor can be used to adjust the PH value Before the eluent). Dried in vacuo to give crude heparin sodium.

  Fourth, refined: The crude heparin sodium chloride solution with 2% completely dissolved, made of its solubility is about 8% of the solution, in the process can be properly heated to dissolve. The above material night with 5mol / L sodium hydroxide solution finely adjusted PH = 8.0-8.2, warmed to 78-80 degrees, according to each one hundred million units of 0.15-0.2mol / L potassium permanganate solution to the purple no longer Fade is the end of the first oxidation operation; then add a little saturated sodium sulfite solution just fade red is appropriate. After the above liquid is properly cooled,

  Fine filtration time, when the filtrate temperature dropped to 36 degrees, the complex filtration time, the filtrate with a little saturated sodium hydroxide solution to adjust PH = 10.5-11.0, with full stirring, slowly adding a small amount of 3% -5% Hydrogen peroxide solution at 25-27 degrees for the second oxidation, the time is 16-24 hours, when the oxidation process is completed, the feed solution was filtered with a "sterile filter", the filtrate was adjusted with a little hydrochloric acid to PH = 5.8-6.5, add 0.9 times the amount of 95% ethanol, at 5-10 degrees for 24 hours under precipitation conditions. The precipitate was collected, dissolved in a small amount of 10% sodium chloride solution, and then precipitated by adding 3-4 times of 95% ethanol to collect the precipitate (ethanol was recovered, distilled and dehydrated and recycled). Precipitate by anhydrous ethanol dehydration, fine grinding, and then dehydrated acetone, fine grinding, dehydration by acetone, far-infrared vacuum drying (50-60 degrees), to obtain heparin sodium.